Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask

Ana Doblas; Ananya Dutta; Genaro Saavedra; Chrysanthe Preza

doi:10.1117/12.2290528

23 February 2018 Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask

Ana Doblas, Ananya Dutta, Genaro Saavedra, Chrysanthe Preza

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104990F (2018) https://doi.org/10.1117/12.2290528
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Previously, a wavefront encoded (WFE) imaging system implemented using a squared cubic (SQUBIC) phase mask has been verified to reduce the sensitivity of the imaging system to spherical aberration (SA). The strength of the SQUBIC phase mask and, as consequence, the performance of the WFE system are controlled by a design parameter, A. Although the higher the A-value, the more tolerant the WFE system is to SA, this is accomplished at the expense of the effective imaging resolution. In this contribution, we investigate this tradeoff in order to find an optimal A-value to balance the effect of SA and loss of resolution.

Citation Download Citation

Ana Doblas, Ananya Dutta, Genaro Saavedra, and Chrysanthe Preza "Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104990F (23 February 2018); https://doi.org/10.1117/12.2290528

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