Label-free histopathological assessment of tissues with photoacoustic remote sensing (PARS) microscopy

Benjamin R. Ecclestone; Saad Abbasi; Deepak Dinakaran; Muba Taher; Kevan Bell; Frank K. H. van Landeghem; John R. Mackey; Parsin Haji Reza

doi:10.1117/12.2577914

5 March 2021 Label-free histopathological assessment of tissues with photoacoustic remote sensing (PARS) microscopy

Benjamin R. Ecclestone, Saad Abbasi, Deepak Dinakaran, Muba Taher, Kevan Bell, Frank K. H. van Landeghem, John R. Mackey, Parsin Haji Reza

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Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550E (2021) https://doi.org/10.1117/12.2577914
Event: SPIE BiOS, 2021, Online Only

Abstract

Here we explore the use of Photoacoustic Remote Sensing (PARS™) microscopy, a recently developed non-contact photoacoustic imaging modality, for visualizing subcellular structures label-free in tissues. Operating in an all-optical reflection-mode architecture PARS captures optical absorption contrast within bulk tissue samples. Presented here, by visualizing endogenous optical absorption of DNA and cytochromes, cellular morphology is captured with contrast analogous to the industry standard hematoxylin and eosin (H&E) staining. Subcellular features are recovered from human and murine, brain and gastrointestinal tissues. This work represents a significant step towards the development of a real-time microscopy system for label-free histopathological assessment of tissues in-situ.

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Benjamin R. Ecclestone, Saad Abbasi, Deepak Dinakaran, Muba Taher, Kevan Bell, Frank K. H. van Landeghem, John R. Mackey, and Parsin Haji Reza "Label-free histopathological assessment of tissues with photoacoustic remote sensing (PARS) microscopy", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550E (5 March 2021); https://doi.org/10.1117/12.2577914

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KEYWORDS

Tissues

Photoacoustic spectroscopy

Microscopy

Remote sensing

Acoustics

Pulsed laser operation

Absorption

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