Bioaerosol detection technology and experimental research based on laser-induced fluorescence

Yi Cai; Chidong Xu; Dongsheng Yu; Zhanying Zhang; Yanlei Li; Weikai Fang; Zhe Yang

doi:10.1117/12.2638359

15 November 2022 Bioaerosol detection technology and experimental research based on laser-induced fluorescence

Yi Cai, Chidong Xu, Dongsheng Yu, Zhanying Zhang, Yanlei Li, Weikai Fang, Zhe Yang

Proceedings Volume 12448, 5th Optics Young Scientist Summit (OYSS 2022); 1244812 (2022) https://doi.org/10.1117/12.2638359
Event: 5th Optics Young Scientist Summit (OYSS 2022), 2022, Fuzhou, China

Abstract

Bioaerosol aerosols originate from a biological source or a biologically active composition in the air everywhere, even in the presence of the ocean and polar regions. Bioaerosols are essential for research in many fields, such as public health safety, botany, and biological warfare. Laser-induced fluorescence technology is a method of using lasers to irradiate a medium so that the electrons in the medium that are originally in equilibrium absorb the photon energy of a specific wavelength and transition to different vibrational energy levels in the excited state, thereby emitting fluorescence of different wavelengths. The wavelength of fluorescence is always longer than the excitation wavelength. In this paper, a bioaerosol detection lidar is developed based on laser-induced fluorescence technology, which can perform fluorescence detection of biological substances at a certain distance, providing a certain basis for real-time detection of bioaerosols. The bioaerosol detection lidar developed in this study provides two laser wavelengths of 266 nm and 355 nm as excitation wavelengths for the detection of two fluorescent substances, tryptophan and riboflavin, contained in bioaerosols. In an outdoor environment, the lidar developed this time was used to verify the detection of tryptophan and riboflavin at different detection distances (30 m, 360 m, 1374 m). After analyzing the detection results, it is concluded that lidar can effectively detect two fluorescent substances, riboflavin and tryptophan, at 30 m, 360 m, and 1374 m.

Citation Download Citation

Yi Cai, Chidong Xu, Dongsheng Yu, Zhanying Zhang, Yanlei Li, Weikai Fang, and Zhe Yang "Bioaerosol detection technology and experimental research based on laser-induced fluorescence", Proc. SPIE 12448, 5th Optics Young Scientist Summit (OYSS 2022), 1244812 (15 November 2022); https://doi.org/10.1117/12.2638359

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KEYWORDS

Laser induced fluorescence

Luminescence

LIDAR

Fluorescence spectroscopy

Aerosols

Atmospheric laser remote sensing

Biological detection systems

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