Combined optical coherence microscopy and confocal microscopy of a 3D, co-culture model of aortic valve calcification

Santosh Balakrishnan; Meg McFetridge; Katherine M. Driscoll; Yuechuan Lin; Nichaluk Leartprapun; Jonathan T. Butcher; Steven G. Adie

doi:10.1117/12.3001787

13 March 2024 Combined optical coherence microscopy and confocal microscopy of a 3D, co-culture model of aortic valve calcification

Santosh Balakrishnan, Meg McFetridge, Katherine M. Driscoll, Yuechuan Lin, Nichaluk Leartprapun, Jonathan T. Butcher, Steven G. Adie

Author Affiliations +

Proceedings Volume 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024; 128190T (2024) https://doi.org/10.1117/12.3001787
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Longitudinal imaging of a 3D model of calcific aortic valve disease, which consisted of co-cultured GFP+ Valve Endothelial Cells (VEC) and Valve Interstitial Cells (VIC), was performed with a combined Optical Coherence Microscopy (OCM), confocal reflectance and fluorescence microscopy system. The acquired confocal volumes depicted the VEC morphological changes and migration as well as collagen fiber alignment. With the aid of computational refocusing and multi-volume processing, the OCM datasets could visualize VIC cell bodies, matrix remodeling, nodule formation and calcific deposits. The complementary information derived using this combined approach could help unravel the cellular mechanisms leading to aortic valve calcification.

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Santosh Balakrishnan, Meg McFetridge, Katherine M. Driscoll, Yuechuan Lin, Nichaluk Leartprapun, Jonathan T. Butcher, and Steven G. Adie "Combined optical coherence microscopy and confocal microscopy of a 3D, co-culture model of aortic valve calcification", Proc. SPIE 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024, 128190T (13 March 2024); https://doi.org/10.1117/12.3001787

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