Novel sequential RGB+NIR fluorescence lifetime imaging with a single nanosecond time-gated CAPS camera

Pooria Iranian; Wannes W. Nevens; Thomas Lapauw; Thomas Van den Dries; Jan Coosemans; Sevada Sahakian; Théo Lepoutte; Valéry A. Jacobs; Hans Ingelberts; Maarten Kuijk

doi:10.1117/12.3000970

12 March 2024 Novel sequential RGB+NIR fluorescence lifetime imaging with a single nanosecond time-gated CAPS camera

Pooria Iranian, Wannes W. Nevens, Thomas Lapauw, Thomas Van den Dries, Jan Coosemans, Sevada Sahakian, Théo Lepoutte, Valéry A. Jacobs, Hans Ingelberts, Maarten Kuijk

Author Affiliations +

Proceedings Volume 12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII; 128310B (2024) https://doi.org/10.1117/12.3000970
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescence imaging is widely used in many different domains, one of which is fluorescence-guided surgery. The temporal behavior called fluorescence lifetime gives deeper insights, making it an additional tool. Current fluorescence-guided surgery imaging typically utilizes two different cameras for natural color and NIR fluorescence imaging, making the system complex and expensive. We propose an alternative approach of sequential RGB+NIR fluorescence lifetime imaging and overlaying the images on each other. Sequential RGB+NIR fluorescence imaging is achieved with a high-speed time-gated camera combined with an RGB LED ring illumination for natural color imaging and a picosecond NIR pulsed laser source for fluorescence lifetime imaging.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Pooria Iranian, Wannes W. Nevens, Thomas Lapauw, Thomas Van den Dries, Jan Coosemans, Sevada Sahakian, Théo Lepoutte, Valéry A. Jacobs, Hans Ingelberts, and Maarten Kuijk "Novel sequential RGB+NIR fluorescence lifetime imaging with a single nanosecond time-gated CAPS camera", Proc. SPIE 12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII, 128310B (12 March 2024); https://doi.org/10.1117/12.3000970

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available