Use of photoproteins for in-vivo functional imaging

Christopher H. Contag

doi:10.1117/12.351018

2 July 1999 Use of photoproteins for in-vivo functional imaging

Christopher H. Contag

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351018
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The relative opacity of mammalian tissue permits the transmission of light from internal biological light sources in small laboratory animals. As such internally expressed bioluminescence can be detected externally revealing spatiotemporal information about tagged biological functions. Enzymes that emit light, photoproteins, have been characterized photoproteins have been used as reporters in a variety of in vitro and ex vivo assays and are now being employed as sources of internal biological light that can be eternally monitored in living animals. Using this approach, spatiotemporal changes in patterns of gene expression, infectious disease and tumor cell growth can be revealed in real time. Monitoring light emissions from internal sources provides a powerful method for cellular and molecular analyses in living animals. This approach is particularly well suited for the evaluation of potential therapeutics including the efficacy of novel DNA-based therapies and vaccines.

Citation Download Citation

Christopher H. Contag "Use of photoproteins for in-vivo functional imaging", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351018

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