Spectral domain phase microscopy: a new tool for measuring cellular dynamics and cytoplasmic flow

Emily J. McDowell; Michael A. Choma; Audrey K. Ellerbee; Joseph A. Izatt

doi:10.1117/12.601505

24 March 2005 Spectral domain phase microscopy: a new tool for measuring cellular dynamics and cytoplasmic flow

Emily J. McDowell, Michael A. Choma, Audrey K. Ellerbee, Joseph A. Izatt

Proceedings Volume 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII; (2005) https://doi.org/10.1117/12.601505
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved interferometric phase information via coherence gating. Here a phase sensitive technique called spectral domain phase microscopy (SDPM) is presented. SDPM is a functional extension of spectral domain optical coherence tomography that allows for the detection of cellular motions and dynamics with nanometer-scale sensitivity. This sensitivity is made possible by the inherent phase stability of spectral domain OCT combined with common-path interferometry. The theory that underlies this technique is presented, the sensitivity of the technique is demonstrated by the measurement of the thermal expansion coefficient of borosilicate glass, and the response of an Amoeba proteus to puncture of its cell membrane is measured. We also exploit the phase stability of SDPM to perform Doppler flow imaging of cytoplasmic streaming in A. proteus. We show reversal of cytoplasmic flow in response to stimuli, and we show that the cytoplasmic flow is laminar (i.e. parabolic) in nature. We are currently investigating the use of SDPM in a variety of different cell types.

Citation Download Citation

Emily J. McDowell, Michael A. Choma, Audrey K. Ellerbee, and Joseph A. Izatt "Spectral domain phase microscopy: a new tool for measuring cellular dynamics and cytoplasmic flow", Proc. SPIE 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII, (24 March 2005); https://doi.org/10.1117/12.601505

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