Application of colloidal semiconductor quantum dots as fluorescent labels for diagnosis of brain glial cancer

Patrícia M. A. Farias; Beate S. Santos; Frederico D. Menezes; Ricardo Ferreira; Fernando J. M. Oliveira Jr.; Hernandes F. Carvalho; Luciana Romão; Vivaldo Moura-Neto; Jane C. O. F. Amaral; Adriana Fontes; Carlos L. Cesar

doi:10.1117/12.646866

27 March 2006 Application of colloidal semiconductor quantum dots as fluorescent labels for diagnosis of brain glial cancer

Patrícia M. A. Farias, Beate S. Santos, Frederico D. Menezes, Ricardo Ferreira, Fernando J. M. Oliveira Jr., Hernandes F. Carvalho, Luciana Romão, Vivaldo Moura-Neto, Jane C. O. F. Amaral, Adriana Fontes, Carlos L. Cesar

Author Affiliations +

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60961B (2006) https://doi.org/10.1117/12.646866
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In this work we present the preparation, characterization and conjugation of colloidal core shell CdS-Cd(OH)₂ quantum dots to health and cancer glial rats living cells in culture media. The particles were obtained via colloidal synthesis in aqueous medium, with final pH=7.3-7.4. Laser Scan Confocal Microscopy (LSCM) and Fluorescence Microscopy were used to evaluate fluorescence intensities and patterns of health and cancer (glioblastoma) glial cells labeled with the quantum dots in different time intervals. Health and cancer glial cells clearly differ in their fluorescence intensities and patterns. These different fluorescence intensities and patterns may be associated to differences concerning cellular membrane and metabolic features of health and cancer cells. The results obtained indicate the potential of the methodology for fast and precise cancer diagnostics.

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Patrícia M. A. Farias, Beate S. Santos, Frederico D. Menezes, Ricardo Ferreira, Fernando J. M. Oliveira Jr., Hernandes F. Carvalho, Luciana Romão, Vivaldo Moura-Neto, Jane C. O. F. Amaral, Adriana Fontes, and Carlos L. Cesar "Application of colloidal semiconductor quantum dots as fluorescent labels for diagnosis of brain glial cancer", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60961B (27 March 2006); https://doi.org/10.1117/12.646866

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