Hyperspectral bright greenish-yellow fluorescence (BGYF) imaging of aflatoxin contaminated corn kernels

Haibo Yao; Zuzana Hruska; Robert L. Brown; Thomas E. Cleveland

doi:10.1117/12.686217

23 October 2006 Hyperspectral bright greenish-yellow fluorescence (BGYF) imaging of aflatoxin contaminated corn kernels

Haibo Yao, Zuzana Hruska, Robert L. Brown, Thomas E. Cleveland

Proceedings Volume 6381, Optics for Natural Resources, Agriculture, and Foods; 63810B (2006) https://doi.org/10.1117/12.686217
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

Aflatoxin contaminated corn poses a serious threat to both domestic animals and humans, because of its carcinogenic properties. Traditionally, corn kernels have been examined for evidence of bright greenish-yellow fluorescence (BGYF), which is an indication of possible presence of Aspergillus flavus, one of the aflatoxin producing strains of fungi, when illuminated with a high-intensity ultra-violet light. The BGYF test is typically the first step that leads to an in-depth chemical analysis for possible aflatoxin contamination. The objective of the present study was to analyze hyperspectral BGYF response of corn kernels under UVA excitation. The target corn samples were collected from a commercial corn field in 2005 and showed abundant BGYF response. The BGYF positive kernels were manually picked out and imaged under a visible near-infrared hyperspectral imaging system under UV radiation with excitation wavelength centered at 365 nm. Initial results exhibited strong emission spectra with peaks centered from 500 nm to 515 nm wavelength range for BGYF positive kernels. Aflatoxin levels on the BGYF positive and negative corn kernels (used as control) were measured subsequently with high performance liquid chromatography. The mean aflatoxin concentration level was 5114 ppb for the BGYF positive and undetectable for the normal kernels.

Citation Download Citation

Haibo Yao, Zuzana Hruska, Robert L. Brown, and Thomas E. Cleveland "Hyperspectral bright greenish-yellow fluorescence (BGYF) imaging of aflatoxin contaminated corn kernels", Proc. SPIE 6381, Optics for Natural Resources, Agriculture, and Foods, 63810B (23 October 2006); https://doi.org/10.1117/12.686217

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