Near-field optical fluorescence correlation spectroscopy

José Pérez; Michael Herrmann; Dietmar Gradl; Andreas Naber

doi:10.1117/12.842793

25 February 2010 Near-field optical fluorescence correlation spectroscopy

José Pérez, Michael Herrmann, Dietmar Gradl, Andreas Naber

Proceedings Volume 7571, Single Molecule Spectroscopy and Imaging III; 757118 (2010) https://doi.org/10.1117/12.842793
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The application of Fluorescence Correlation Spectroscopy (FCS) to Near-field Scanning Optical Microscopy on diffusing molecules in solution is presented here. The ultra small excitation volume in such a technique allows measurements with fluorophore concentrations of up to 10μM, making FCS in biological environment at nearly native conditions possible.

Citation Download Citation

José Pérez, Michael Herrmann, Dietmar Gradl, and Andreas Naber "Near-field optical fluorescence correlation spectroscopy", Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 757118 (25 February 2010); https://doi.org/10.1117/12.842793

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