Low power laser and LED irradiation effect on proliferation and differentiation of Wistar rats mesenchymal stem cells

Diana Mancera; Efrain Solarte; Leonardo Fierro; William Criollo

doi:10.1117/12.2021766

18 November 2013 Low power laser and LED irradiation effect on proliferation and differentiation of Wistar rats mesenchymal stem cells

Diana Mancera, Efrain Solarte, Leonardo Fierro, William Criollo

Author Affiliations +

Proceedings Volume 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications; 8785DY (2013) https://doi.org/10.1117/12.2021766
Event: 8th Ibero American Optics Meeting/11th Latin American Meeting on Optics, Lasers, and Applications, 2013, Porto, Portugal

Abstract

It has been demonstrated that appropriately cultured and stimulated mesenchymal cells, can give rise to cells of all tissues of the body. We evaluate the cell proliferation and differentiation induced by low power light irradiation in cell cultures of mesenchymal cells, isolated and previously characterized, from Wistar rats. Roche® XTT and LDH tests were used to assess proliferation and cytotoxicity. Cellular differentiation was determined by optical microscopy and using specific fluorescent markers. We report laser cellular proliferation enhancement by 532 and 473 nm, and the best cell culture response by a dose of 2 Jcm^-2. Although a three day irradiation protocol the cultures grown and no cytotoxicity was detected. Cellular differentiation occurred, and the production of cardiomyocytes was promoted by the cell proliferation stimulated by low power laser irradiation.

Citation Download Citation

Diana Mancera, Efrain Solarte, Leonardo Fierro, and William Criollo "Low power laser and LED irradiation effect on proliferation and differentiation of Wistar rats mesenchymal stem cells", Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785DY (18 November 2013); https://doi.org/10.1117/12.2021766

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