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27 April 2016 Restoration of STORM images from sparse subset of localizations (Conference Presentation)
Alexander A. Moiseev, Grigory V. Gelikonov, Valentine M. Gelikonov
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Proceedings Volume 9714, Single Molecule Spectroscopy and Superresolution Imaging IX; 97140O (2016) https://doi.org/10.1117/12.2212825
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
To construct a Stochastic Optical Reconstruction Microscopy (STORM) image one should collect sufficient number of localized fluorophores to satisfy Nyquist criterion. This requirement limits time resolution of the method. In this work we propose a probabalistic approach to construct STORM images from a subset of localized fluorophores 3-4 times sparser than required from Nyquist criterion. Using a set of STORM images constructed from number of localizations sufficient for Nyquist criterion we derive a model which allows us to predict the probability for every location to be occupied by a fluorophore at the end of hypothetical acquisition, having as an input parameters distribution of already localized fluorophores in the proximity of this location. We show that probability map obtained from number of fluorophores 3-4 times less than required by Nyquist criterion may be used as superresolution image itself. Thus we are able to construct STORM image from a subset of localized fluorophores 3-4 times sparser than required from Nyquist criterion, proportionaly decreasing STORM data acquisition time. This method may be used complementary with other approaches desined for increasing STORM time resolution.
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Alexander A. Moiseev, Grigory V. Gelikonov, and Valentine M. Gelikonov "Restoration of STORM images from sparse subset of localizations (Conference Presentation)", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140O (27 April 2016); https://doi.org/10.1117/12.2212825
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KEYWORDS
Super resolution
Data acquisition
Imaging spectroscopy
Microscopy
Optical microscopy
Stochastic processes
Applied physics
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