Image analysis of topical drug uptake in tissue after ablative fractional laser treatment

Haley L. Marks; Sarfaraz A. Quadri; Joshua Glahn; Michael Wang-Evers; James Talton; Dieter Manstein

doi:10.1117/12.2610180

7 March 2022 Image analysis of topical drug uptake in tissue after ablative fractional laser treatment

Haley L. Marks, Sarfaraz A. Quadri, Joshua Glahn, Michael Wang-Evers, James Talton, Dieter Manstein

Proceedings Volume PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI; PC1193807 (2022) https://doi.org/10.1117/12.2610180
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Ablative fractional laser (AFL) treatment prior to application of a topical drug enhances drug uptake and the resultant coagulation zone (CZ) surrounding each fractional photothermal injury may provide a reservoir for sustained drug release. In this work, we evaluate how morphological changes in the skin after AFL affect the uptake of an intrinsically fluorescent topical antibiotic. Brightfield images of NBTC stained histopathological slides were evaluated using a deep learning approach for semantic segmentation of fractional laser patterns for automatic assessment of laser hole diameter and CZ morphology. Last, collagen denaturation and drug uptake were quantified via polarization and fluorescence microscopy, respectively.

Conference Presentation

Citation Download Citation

Haley L. Marks, Sarfaraz A. Quadri, Joshua Glahn, Michael Wang-Evers, James Talton, and Dieter Manstein "Image analysis of topical drug uptake in tissue after ablative fractional laser treatment", Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC1193807 (7 March 2022); https://doi.org/10.1117/12.2610180

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