Presentation
2 March 2022 Time-lapse Brillouin imaging of the neural tube closure in chick embryo
Author Affiliations +
Abstract
Neural tube closure, or neurulation, has been studied across a range of vertebrates as it is the basis of embryonic development. Closure failure can lead to severe congenital malformations. Current technologies require fixed specimens and physical contact to extract a modulus. Here we investigate the mechanical changes of the neural plate from formation to closure within intact live chick embryos using time-lapse Brillouin imaging and ex-ovo culture. We observed an increase in the Brillouin modulus of the neural plate as the embryo develops in ex-ovo culture. By quantifying the timing and the extent of the forces that drive neural tube closure, we can more accurately identify when and why neural tube defects occur.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Chenchen Handler, Jitao Zhang, and Giuliano Scarcelli "Time-lapse Brillouin imaging of the neural tube closure in chick embryo", Proc. SPIE PC11962, Optical Elastography and Tissue Biomechanics VIV, PC1196207 (2 March 2022); https://doi.org/10.1117/12.2610683
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KEYWORDS
Atomic force microscopy

Mechanics

Organisms

Tissues

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