Disperse scatterer model of OCT and its application to design volumetric differential contrast imaging

Kiriko Tomita; Shuichi Makita; Naoki Fukutake; Rion Morishita; Ibrahim Abd El-Sadek; Pradipta Mukherjee; Antonia Lichtenegger; Junya Tamaoki; Lixuan Bian; Makoto Kobayashi; Tmoko Mori; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.2652325

27 March 2023 Disperse scatterer model of OCT and its application to design volumetric differential contrast imaging

Kiriko Tomita, Shuichi Makita, Naoki Fukutake, Rion Morishita, Ibrahim Abd El-Sadek, Pradipta Mukherjee, Antonia Lichtenegger, Junya Tamaoki, Lixuan Bian, Makoto Kobayashi, Tmoko Mori, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671Q (2023) https://doi.org/10.1117/12.2652325
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Differential interference microscopy (DIC) is a method to obtain the refractive index distribution of a sample as contrast. It is suitable for biological cells, however, DIC can only obtain 2D images from thin samples. Therefore, we introduce a new imaging method, volumetric differential contrast (VDC) imaging using optical coherence tomography (OCT). This method enables getting 3D differential contrast of thick samples. VDC was designed based on the disperse scatterer model (DSM), a new theoretical model of OCT, and obtains differential contrast by complex numerical processing of OCT signal. DSM represents the sample as a spatially distributed refractive index with dispersed random scatterers, and OCT signal was formulated from this model. VDC uses two complex OCT signals, s1 and s2 at two laterally slightly distant positions, and the final image is defined as Im[s1 s2. This signal forms a spatially differential image of the product of the refractive index distribution and the scatterer density. According to the formulation, the size of the differentiation kernel, corresponding to the shear amount of DIC, is proportional to the defocus of the probe beam and the separation between s1 and s2. This method was validated by an in vitro spheroid sample and an in vivo zebrafish sample, measured by spectral domain OCT with a center wavelength of 830 nm. VDC images were obtained from refocused and defocused signals.

Citation Download Citation

Kiriko Tomita, Shuichi Makita, Naoki Fukutake, Rion Morishita, Ibrahim Abd El-Sadek, Pradipta Mukherjee, Antonia Lichtenegger, Junya Tamaoki, Lixuan Bian, Makoto Kobayashi, Tmoko Mori, Satoshi Matsusaka, and Yoshiaki Yasuno "Disperse scatterer model of OCT and its application to design volumetric differential contrast imaging", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671Q (27 March 2023); https://doi.org/10.1117/12.2652325

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available