Biomechanical assessment of murine embryonic neural tube defects using multimodal OCT-Brillouin system (Conference Presentation)

Yogeshwari S. Ambekar; Carlo Donato Caiaffa; Manmohan Singh; Alexander W. Schill; John Steele; Salavat R. Aglyamov; Richard H. Finnell; Giuliano Scarcelli; Kirill V. Larin

doi:10.1117/12.2650173

15 March 2023 Biomechanical assessment of murine embryonic neural tube defects using multimodal OCT-Brillouin system (Conference Presentation)

Yogeshwari S. Ambekar, Carlo Donato Caiaffa, Manmohan Singh, Alexander W. Schill, John Steele, Salavat R. Aglyamov, Richard H. Finnell, Giuliano Scarcelli, Kirill V. Larin

Author Affiliations +

Proceedings Volume PC12381, Optical Elastography and Tissue Biomechanics X; PC123810K (2023) https://doi.org/10.1117/12.2650173
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Neural tube closure is a complex process driven by mechanical forces, but this process can be disturbed leading to development defects. So, to understand the interplay between forces and tissue stiffness during neurulation, we developed a multimodal Brillouin microscopy and optical coherence system (OCT). OCT provides structural guidance while mapping the biomechanical properties of embryonic neural tube using Brillouin microscopy. 3D-OCT, 2D-OCT, and 2D-Brillouin images of Mthfd1l and Fuz knockout mouse embryos at gestation days 9.5 and 10.5 were acquired. Our results show overall decrease in the stiffness of homozygotic knockout neural tube tissues compared to the wildtype.

Conference Presentation

Citation Download Citation

Yogeshwari S. Ambekar, Carlo Donato Caiaffa, Manmohan Singh, Alexander W. Schill, John Steele, Salavat R. Aglyamov, Richard H. Finnell, Giuliano Scarcelli, and Kirill V. Larin "Biomechanical assessment of murine embryonic neural tube defects using multimodal OCT-Brillouin system (Conference Presentation)", Proc. SPIE PC12381, Optical Elastography and Tissue Biomechanics X, PC123810K (15 March 2023); https://doi.org/10.1117/12.2650173

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