Extended depth-of-field microscopy with surface tracking for noninvasive examination of conjunctival goblet cells in humans

Jungbin Lee; Seonghan Kim; Wan Jae Choi; Jin Suk Ryu; Chang Ho Yoon; Soohyun Park; Won Yeong Park; Noseong Park; Suil Jeon; Jieun Yun; Ki Hean Kim

doi:10.1117/12.2687883

13 March 2024 Extended depth-of-field microscopy with surface tracking for noninvasive examination of conjunctival goblet cells in humans

Jungbin Lee, Seonghan Kim, Wan Jae Choi, Jin Suk Ryu, Chang Ho Yoon, Soohyun Park, Won Yeong Park, Noseong Park, Suil Jeon, Jieun Yun, Ki Hean Kim

Author Affiliations +

Proceedings Volume PC12824, Ophthalmic Technologies XXXIV; PC128240S (2024) https://doi.org/10.1117/12.2687883
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Conjunctival goblet cells (CGCs) are specialized epithelial cells that secrete mucins to form the mucous layer of the protective tear film and by suppressing inflammation. Although CGCs are an important biomarker for diagnosing ocular surface diseases, rapid and noninvasive CGC examination methods have not been available. We have developed a new imaging system, high-speed extended depth-of-field wide-field microscopy with surface tracking, to enable non-contact large-area CGC imaging in human subjects. A novel long-range surface detection method was developed for rapid large-area mosaic imaging with lateral translation. Large-area CGC imaging and density quantification in human subjects was demonstrated. This new imaging system could be useful for noninvasive CGC examination in humans, which could be valuable for precision diagnosis and optimal treatment of ocular surface diseases.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jungbin Lee, Seonghan Kim, Wan Jae Choi, Jin Suk Ryu, Chang Ho Yoon, Soohyun Park, Won Yeong Park, Noseong Park, Suil Jeon, Jieun Yun, and Ki Hean Kim "Extended depth-of-field microscopy with surface tracking for noninvasive examination of conjunctival goblet cells in humans", Proc. SPIE PC12824, Ophthalmic Technologies XXXIV, PC128240S (13 March 2024); https://doi.org/10.1117/12.2687883

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KEYWORDS

Depth of field

Microscopy

Diseases and disorders

Human subjects

Eye models

Fluorescence microscopy

Image deconvolution

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