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13 March 2024 Development and translation of an infrared laser scanning microscope for whole-slide chemical imaging
Kevin L. Yeh, Ishaan Sharma, Kianoush Falahkheirkhah, Matthew P. Confer, Andres C. Orr, Yen-Ting Liu, Ruo-Jing Ho, Mykolas Tamonis, John C. Cheville, Rohit Bhargava
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128551A (2024) https://doi.org/10.1117/12.3003524
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Chemical imaging, especially mid-infrared spectroscopic microscopy, enables label-free biomedical analyses while achieving expansive molecular sensitivity. However, its slow speed and poor image quality impede widespread adoption. We present a microscope that provides high-throughput recording, low noise, and high spatial resolution where the bottom-up design of its optical train facilitates dual-axis galvo laser scanning of a diffraction-limited focal point over large areas using custom infinity-corrected objectives. The data quality enables applications of modern machine learning and capabilities not previously feasible. Distinct from conventional approaches that focus on morphological investigations or immunostaining, this development makes label-free imaging of minimally processed tissue practical.
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Kevin L. Yeh, Ishaan Sharma, Kianoush Falahkheirkhah, Matthew P. Confer, Andres C. Orr, Yen-Ting Liu, Ruo-Jing Ho, Mykolas Tamonis, John C. Cheville, and Rohit Bhargava "Development and translation of an infrared laser scanning microscope for whole-slide chemical imaging", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128551A (13 March 2024); https://doi.org/10.1117/12.3003524
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KEYWORDS
Imaging spectroscopy
Microscopes
Biomedical optics
Infrared lasers
Infrared spectroscopy
Chemical analysis
Quantum cascade lasers
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