Ms. Yahui Chen Profile

Yahui Chen

at China Agricultural Univ

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Proceedings Article | 13 June 2023 Poster + Paper

Raman enhancement effect of different Au @ Ag NPs on S. Typhi

Yahui Chen, Yankun Peng, Qinghui Guo, Jianwei Qin

Proceedings Volume 12545, 125450C (2023) https://doi.org/10.1117/12.2663272

KEYWORDS: Silver, Gold, Raman spectroscopy, Surface enhanced Raman spectroscopy, Bacteria, Nanoparticles, Pathogens, Colloids, Raman scattering, Gold nanoparticles

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S. Typhi was a common foodborne pathogen that often enters the human body through food and caused illness. In order to achieve the rapid detection of S. Typhi, surface enhanced Raman spectroscopy (SERS) had been studied by more and more people. To prepare an efficient, stable, and simple signal enhancer, the signal enhancement effects of Au NPs, Ag NPs, and Au @ Ag NPs on S. Typhi were compared. Because of the good enhancement effect and stability, Au @ Ag NPs was used as the research object. The influence of SERS substrates with the same gold core size and different silver shell thickness on bacterial signal enhancement was investigated. Au @ Ag NPs with different thickness silver layers were prepared by changing the concentration of silver nitrate, and colloids were characterized by UV spectroscopy. After de-noising, smoothing and de-baselining of SERS spectra, the characteristic peak intensity information was used to analyze the detection limit and detection stability of S. Typhi. The experimental results showed that when the silver layer thickness was prepared by 4 mM silver nitrate, Au @ Ag NPs had the best amplification effect on S. Typhi Raman signal, which could achieve a detection limit of 104 CFU/mL, and the coefficient of variation of SERS characteristic peak was less than 7%. In addition, the quantitative prediction model of S. Typhi with 10⁵ CFU/mL - 10⁸ CFU/mL was established by MLR, which achieved a correlation coefficient (Rc) of 0.99 and a root mean square error (RMSEC) of 10^0.0364 CFU/mL.

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