The aim of our study was to identify fluorescence excitation-emission pairs correlated with atherosclerotic
pathology in ex-vivo human aorta. Wide-field images of atherosclerotic human aorta were captured using UV and
visible excitation and emission wavelength pairs of several known fluorophores to investigate correspondence with
gross pathologic features. Fluorescence spectroscopy and histology were performed on 21 aortic samples. A matrix
of Pearson correlation coefficients were determined for the relationship between relevant histologic features and the
intensity of emission for 427 wavelength pairs. A multiple linear regression analysis indicated that elastin (370/460
nm) and tryptophan (290/340 nm) fluorescence predicted 58% of the variance in intima thickness (R-squared =
0.588, F(2,18) = 12.8, p=.0003), and 48% of the variance in media thickness (R-squared = 0.483, F(2,18) = 8.42,
p=.002), suggesting that endogenous fluorescence intensity at these wavelengths can be utilized for improved
pathologic characterization of atherosclerotic plaques.
William Lewis, Maura Williams, and Walfre Franco, "Ex-vivo UV autofluorescence imaging and fluorescence spectroscopy of atherosclerotic pathology in human aorta," Proc. SPIE 10042, Diagnostic and Therapeutic Applications of Light in Cardiology, 100420Q (Presented at SPIE BiOS: January 29, 2017; Published: 8 February 2017); https://doi.org/10.1117/12.2252590.
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