Photodynamic therapy (PDT) has emerged as an effective treatment modality for various malignant neoplasia
and diseases. In PDT, the photochemical interaction of photosensitizer (PS), light and molecular oxygen
produces singlet oxygen which can lead to tumour cell apoptosis, necrosis or autophagy. The success of PDT
is limited by the hydrophobic characteristic of the PS which hinders treatment administration and efficiency.
To circumvent this limitation, PS can be incorporated in nanostructured drug delivery systems such as gold
nanoparticles (AuNPs). In this study, we investigated the effectiveness of free zinc monocarboxyphenoxy
phthalocyanine (ZnMCPPc) and ZnMCPPc conjugated to AuNPs. Commercially purchased melanoma cancer
cells cultured as cell monolayers were used in this study. Changes in cellular response were evaluated using
cellular morphology, viability, proliferation and cytotoxicity. Untreated cells showed no changes in cellular
morphology, proliferation and cytotoxicity. However, photoactivated free ZnMCPPc and ZnMCPPc conjugated
to AuNPs showed changes in cellular morphology and a dose dependent decrease in cellular viability and
proliferation as well as an increase in cell membrane. ZnMCPPc conjugated to AuNPs showed an improved
efficiency in PDT as compared to free ZnMCPPc, which might be as a result of the vehicle effect of AuNPs.
Both PSs used in this study were effective in inducing cell death with ZnMCPPc conjugated to AuNPs
showing great potential as an effective PS for PDT.
Sello Lebohang Manoto, David Oluyinka Oluwole, Rudzani Malabi, Charles Maphanga, Saturnin Ombinda-Lemboumba, Tebello Nyokong, and Patience Mthunzi-Kufa, "Phototoxic effects of free phthalocyanine and phthalocyanine conjugated to gold nanoparticles for targeted photodynamic therapy of melanoma cancer," Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 100470D (Presented at SPIE BiOS: January 28, 2017; Published: 8 February 2017); https://doi.org/10.1117/12.2252682.
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Monte Carlo based light propagation models to improve efficacy of biophotonics based therapeutics of hollow organs and solid tumours including photodynamic therapy and photobiomodulation (Conference Presentation)