We developed a three-photon adaptive optics add-on to a commercial two-photon laser scanning microscope. We
demonstrated its capability for structural and functional imaging of neurons labeled with genetically encoded red
fluorescent proteins or calcium indicators deep in the living mouse brain with cellular and subcellular resolution.
Xiaodong Tao, Ju Lu, Tuwin Lam, Ramiro Rodriguez, Yi Zuo, and Joel Kubby, "A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging," Proc. SPIE 10051, Neural Imaging and Sensing, 100510R (Presented at SPIE BiOS: January 31, 2017; Published: 8 February 2017); https://doi.org/10.1117/12.2253922.
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