Optical coherence tomography (OCT) enables non-invasive, high-resolution, tomographic imaging of biological tissues by leveraging principles of low coherence interferometry; however, OCT lacks molecular specificity. Spectroscopic OCT (SOCT) overcomes this limitation by providing depth-resolved spectroscopic signatures of chromophores, but SOCT has been limited to a couple of endogenous molecules, namely hemoglobin and melanin. Stimulated Raman scattering, on the other hand, can provide highly specific molecular information of many endogenous species, but lacks the spatial and spectral multiplexing capabilities of SOCT. In this work we integrate the two methods, SRS and SOCT, to enable simultaneously multiplexed spatial and spectral imaging with sensitivity to many endogenous biochemical species that play an important role in biology and medicine. The method, termed SRS-SOCT, has the potential to achieve fast, volumetric, and highly sensitive label-free molecular imaging, which would be valuable for many applications. We demonstrate the approach by imaging excised human adipose tissue and detecting the lipids’ Raman signatures in the high-wavenumber region. Details of this method along with validations and results will be presented.
Francisco E. Robles, Kevin C. Zhou, Martin C. Fischer, and Warren S. Warren, "Stimulated Raman scattering (SRS) spectroscopic OCT (Conference Presentation)," Proc. SPIE 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, 100530X (Presented at SPIE BiOS: January 31, 2017; Published: 19 April 2017); https://doi.org/10.1117/12.2254829.5371723146001.
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