Optical coherence tomography (OCT) retinal imaging contributes to understanding central nervous system (CNS)
diseases because the eye is an anatomical “window to the brain” with direct optical access to nonmylenated retinal
ganglion cells. However, many CNS diseases are associated with neuronal changes beyond the resolution of standard
OCT retinal imaging systems. Though studies have shown the utility of scattering angle resolved (SAR) OCT for particle
sizing and detecting disease states ex vivo, a compact SAR-OCT system for in vivo rodent retinal imaging has not
previously been reported. We report a fiber-based SAR-OCT system (swept source at 1310 nm ± 65 nm, 100 kHz scan
rate) for mouse retinal imaging with a partial glass window (center aperture) for angular discrimination of backscattered
light. This design incorporates a dual-axis MEMS mirror conjugate to the ocular pupil plane and a high collection
efficiency objective. A muring retina is imaged during euthanasia, and the proposed SAR-index is examined versus time.
Results show a positive correlation between the SAR-index and the sub-cellular hypoxic response of neurons to
isoflurane overdose during euthanasia. The proposed SAR-OCT design and image process technique offer a contrast
mechanism able to detect sub-resolution neuronal changes for murine retinal imaging.
Michael R. Gardner, Nitesh Katta, Austin McElroy, Vikram Baruah, H. Grady Rylander, and Thomas E. Milner, "Scattering angle resolved optical coherence tomography for in vivo murine retinal imaging," Proc. SPIE 10053, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, 100531O (Presented at SPIE BiOS: February 01, 2017; Published: 17 February 2017); https://doi.org/10.1117/12.2253367.
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