Endogenous fluorescence is a powerful technique for probing both structure and function of tissue. We show that enabling wide-field fluorescence microscopy with chemometrics can significantly enhance the performance of tissue diagnosis with endogenous fluorescence. The spatial distribution and absolute concentration of fluorophores is uncovered with non-negative factorization aided by the spatial diversity from microscopic autofluorescence color images. Fluorescence quantification in terms of its absolute concentration map avoids issues dependent on specific measurement approach or systems and yields biologically meaningful data. The standardization of endogenous fluorescence in terms of absolute concentration will facilitate its translation to the clinics and simplifies the assessment of competing methods relating to tissue fluorescence.
Run Li, Kevin Vasquez, and M. Xu, "Chemometric endogenous fluorescence for tissue diagnosis," Proc. SPIE 10060, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1006017 (Presented at SPIE BiOS: February 02, 2017; Published: 17 February 2017); https://doi.org/10.1117/12.2255615.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the conference proceedings. They include the speaker's narration along with a video recording of the presentation slides and animations. Many conference presentations also include full-text papers. Search and browse our growing collection of more than 12,000 conference presentations, including many plenary and keynote presentations.