The tissue optical clearing technique could significantly enhance the biomedical optical imaging depth, but current investigations are mainly limited to in vitro studies. In vivo tissue optical clearing method should be enough rapid, transparent and safe, which makes it more difficult, especially, for hard tissue. During the past years, we developed skull optical clearing methods for in vivo cortical imaging. This presentation will report recent progress in skull optical clearing method, including their efficacy, safety, and applications. The skull optical clearing method is proved to be effective for adult mice ages in different month and permit various imaging techniques to monitor cortical blood flow, blood oxygen, and vascular with high resolution and contrast, not only for local cortex, but also for whole cortex. The long-term and short-term observation show that there is no obvious effect on cortical vascular function when laser speckle contrast imaging and hyperspectral imaging are used to repeatedly image the cortical blood flow, blood oxygen. Finally, we will demonstrate some applications for physiological or pathological situation, including monitoring the anoxia, drug-induced cortical response, et al.
Dan Zhu, "Skull optical clearing for assessing to cerebral hemodynamics with high contrast and resolution (Conference Presentation)," Proc. SPIE 10063, Dynamics and Fluctuations in Biomedical Photonics XIV, 100630K (Presented at SPIE BiOS: January 30, 2017; Published: 19 April 2017); https://doi.org/10.1117/12.2253508.5371893406001.
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