Fluorescence of Protein has been widely used in diagnostic oncology for characterizing cellular metabolism. However, the intensity of fluorescence emission is affected due to the absorbers and scatterers in tissue, which may lead to error in estimating exact protein content in tissue. Extraction of intrinsic fluorescence from measured fluorescence has been achieved by different methods. Among them, Monte Carlo based method yields the highest accuracy for extracting intrinsic fluorescence. In this work, we have attempted to generate a lookup table for Monte Carlo simulation of fluorescence emission by protein. Furthermore, we fitted the generated lookup table using an empirical relation. The empirical relation between measured and intrinsic fluorescence is validated using tissue phantom experiments. The proposed relation can be used for estimating intrinsic fluorescence of protein for real-time diagnostic applications and thereby improving the clinical interpretation of fluorescence spectroscopic data.
Gnanatheepam Einstein, Kanniyappan Udayakumar, Prakasarao Aruna, and Singaravelu Ganesan, "Intrinsic fluorescence of protein in turbid media using empirical relation based on Monte Carlo lookup table," Proc. SPIE 10063, Dynamics and Fluctuations in Biomedical Photonics XIV, 100630N (Presented at SPIE BiOS: January 30, 2017; Published: 3 March 2017); https://doi.org/10.1117/12.2255883.
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