We have recently developed Laser Immunotherapy (LIT), a targeted cancer treatment modality using synergistic application of near-infrared laser irradiation and in situ immunological stimulation. This study further investigates the principles underlying the immune response to LIT treatment by studying immunological impact of the laser photothermal effect in vivo, in vitro, and ex vivo. Tumor cells were stressed in vitro, and samples were collected to analyze protein expression with a Western Blot. Additionally, a tumor model was designed using bovine liver tissue suspended in agarose gel which was treated using laser interstitially and monitored with both proton-resonance frequency shift MR thermometry and thermocouples. From the bovine liver tumor model, we were able to develop the correlation between tissue temperature elevation and laser power and distance from the fiber tip. Similar data was collected by monitoring the temperature of a metastatic mammary tumor in a rat during laser irradiation. Ultimately, these results show that the laser irradiation of LIT leads to clear immunological effects for an effective combination therapy to treat metastatic cancers.
Austin Doughty, Shaojie Liu, Feifan Zhou, Hong Liu, and Wei R. Chen, "Development of ex vivo model for determining temperature distribution in tumor tissue during photothermal therapy (Conference Presentation)," Proc. SPIE 10065, Biophotonics and Immune Responses XII, 100650B (Presented at SPIE BiOS: January 30, 2017; Published: 19 April 2017); https://doi.org/10.1117/12.2250395.5379284530001.
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