We demonstrate ultrafast time-stretch microscopy in, to the best of our knowledge, the shortest wavelength regimes, i.e. 532 nm. This is enabled by a new all-optical ultrahigh-speed laser-scanning technique called free-space angular-chirpenhanced delay (FACED) that achieves a line-scan rate as high as 20 MHz. In contrast to the predominant fiber-based implementation, time-stretch imaging based on FACED allows wavelength-independent and low-loss operations, and more intriguingly reconfigurable all-optical laser-scanning rate. Using this technique, we present high-resolution single-cell images captured in an ultrafast microfluidic flow (1.5m/s). This could unleash numerous cell and tissue imaging applications, e.g. high-throughput image flow cytometry and whole-slide imaging.
Wenwei Yan, Jianglai Wu, Kenneth K. Y. Wong, and Kevin K. Tsia, "Ultrafast, laser-scanning time-stretch microscopy with visible light," Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681C (Presented at SPIE BiOS: February 01, 2017; Published: 16 February 2017); https://doi.org/10.1117/12.2251094.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for 2018 presentations, with transcripts for prior recordings added daily.
Search our growing collection of more than 16,000 conference presentations, including many plenaries and keynotes.