Tendon rupture is a trauma that is difficult to fully recover from. Therefore, non-destructive and non-invasive evaluation method for the tendon healing is strongly required. In this study, we performed the orientation analysis of collagen fiber in healing tendon by two-dimensional Fourier transform (2D-FT) of SHG image. The extracted 2D-FT power spectra imply the correlation with the degree of the tendon healing. These results indicate that SHG microscopy has a unique potential as a non-destructive and non-invasive indicator of tendon healing.
E. Hase, T. Minamikawa, K. Sato, D. Yonekura, M. Takahashi, and T. Yasui, "Orientation analysis of collagen fibers in healing tendon by using second-harmonic-generation microscopy," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006909 (Presented at SPIE BiOS: January 29, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2251111.
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