We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. We demonstrate an imaging depth of 1,200 μm in vasculature and 1,160 μm in neurons. We also demonstrate deep-tissue imaging using Indocyanine Green (ICG), which is FDA approved and a promising route to translate multiphoton microscopy to human applications.
David R. Miller, Flor A. Medina, Ahmed Hassan, Evan P. Perillo, Kristen Hagan, S. M. Shams Kazmi, Boris V. Zemelman, and Andrew K. Dunn, "In vivo multiphoton microscopy beyond 1 mm in the brain," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690C (Presented at SPIE BiOS: January 29, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2249949.
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