A range of proposed alkyne Raman tags are examined in-silico for activity and then synthesised generating a library of analogues of the natural product, anisomycin. We report the use of bisaryl butadiyne-anisomycin (BADY-anisomycin) in intracellular SRS microscopy studies of uptake and localisation within live and fixed cells. Following rational design and synthesis, BADY-anisomycin was shown to produce an intense Raman band at 2219 cm-1, that is centrally located within the cellular silent region and is approximately 60 times more Raman active than the corresponding propargylanisomycin. Finally, we demonstrate two-colour imaging utilising EdU, an alkyne-containing proliferation probe and BADY-anisomycin.
M. Lee, W. J. Tipping, A. Serrels, A. N. Hulme, and V. G. Brunton, "New approaches to intracellular drug imaging by stimulated Raman scattering microscopy," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690T (Presented at SPIE BiOS: January 30, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2253847.
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