We demonstrate a robust, all-fiber, two-wavelength time-lens source for background-free coherent anti-Stokes Raman scattering (CARS) imaging. The time-lens source generates two picosecond pulse trains simultaneously: one at 1064 nm and the other tunable between 1040 nm and 1075 nm (~ 400 mW for each wavelength). When synchronized to a modelocked Ti:Sa laser, the two wavelengths are used to obtain on- and off-resonance CARS images. Real-time subtraction of the nonresonant background in the CARS image is achieved by the synchronization of the pixel clock and the time-lens source. Background-free CARS imaging of sebaceous glands in ex vivo mouse tissue is demonstrated.
Bo Li, Kriti Charan, Ke Wang, David Sinefeld, and Chris Xu, "Multi-wavelength time-lens source and its application to nonresonant background suppression for coherent anti-Stokes Raman scattering microscopy," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690Y (Presented at SPIE BiOS: January 30, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2250347.
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