We have previously demonstrated a total internal reflection, wide-field CARS microscope, where the signal is enhanced with the aid of a thin gold layer that supports surface plasmon polariton resonances. This surface-enhanced CARS microscope is capable of generating images of lipid structures in close proximity (<100 nm) to the glass substrate at excitation densities that are 4 orders of magnitude lower than in point-scanning CARS microscopy. In this contribution, we demonstrate its application to visualizing lipids in aqueous media, including imaging of cells, with a unique surface-sensitive contrast that cannot be obtained with conventional CARS microscopy.
Alexander Fast, John T. Kenison, and Eric O. Potma, "Wide-field surface-enhanced CARS microscopy of cells (Conference Presentation)," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006910 (Presented at SPIE BiOS: January 30, 2017; Published: 24 April 2017); https://doi.org/10.1117/12.2254549.5380018896001.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the conference proceedings. They include the speaker's narration along with a video recording of the presentation slides and animations. Many conference presentations also include full-text papers. Search and browse our growing collection of more than 12,000 conference presentations, including many plenary and keynote presentations.