Stimulated Raman scattering (SRS) microscopy is a promising technique for label-free imaging of living systems. We demonstrate microsecond-scale SRS spectral imaging by tuning two spectrally focused pulses temporally through a resonant delay-line. Our platform acquired an SRS spectrum within 42 microseconds and formed a spectral image composed of 40,000 pixels in real-time.
Chien-Sheng Liao, Kai-Chih Huang, Weili Hong, Andy J. Chen, Caroline Karanja, Pu Wang, Gregory Eakins, and Ji-Xin Cheng, "Stimulated Raman spectroscopic imaging by microsecond delay-line tuning," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006911 (Presented at SPIE BiOS: January 31, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2256802.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the conference proceedings. They include the speaker's narration along with a video recording of the presentation slides and animations. Many conference presentations also include full-text papers. Search and browse our growing collection of more than 12,000 conference presentations, including many plenary and keynote presentations.