A novel therapeutic drug monitoring point of care testing (POCT) optical device for the detection of immunosuppressants in transplanted patients was designed and tested, with the body interface constituted by an intravascular microdialysis catheter (MicroEye®) which provides the dialysate as clinical sample. An optical biochip with 10 microchannels, based on total internal reflection fluorescence (TIRF), enables the frequent measurement of immunosuppressants. Heterogeneous competitive immunoassays for the detection of mycophenolic acid, tacrolimus and cyclosporine A are implemented on the different microchannels, with the derivative of the immunosuppressants immobilised on the bottom part of the micro-channels.
Francesco Baldini, Chiara Berrettoni, Ambra Giannetti, Sara Tombelli, Cosimo Trono, Giampiero Porro, Romeo Bernini, Immacolata Angelica Grimaldi, Genni Testa, Gianluca Persichetti, Claudia Gärtner, Holger Becker, Marcel Berner, Markus B. Schubert, Mark T. O'Connell, Daniel Carney, Guillermo Orellana, Ana B. Descalzo, Francesca Salis, Paulo P. Freitas, Peter B Luppa, Heike Bittersohl, Günter Gauglitz, Urs Hilbig, and Kathrin Freudenberger, "Novel fluorescence-based POCT platform for therapeutic drug monitoring in transplanted patients (Conference Presentation)," Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720C (Presented at SPIE BiOS: January 30, 2017; Published: 24 April 2017); https://doi.org/10.1117/12.2255623.5380600071001.
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