Quantitative phase-gradient or phase imaging in LED microscopy has been recently demonstrated. The methods enable measurement of phase distribution of transparent specimens in a simple and cost-effective manner, but require multiple image acquisitions with different source or pupil configurations to improve phase accuracy.
Here, we demonstrate a strategy for single-shot quantitative phase imaging in color-coded LED microscopy. We employ a circular LED illumination pattern that is trisected into subregions with equal area, assigned to red, green and blue colors, respectively. Additional color filter is also employed to mitigate the color leakage of light into different color channels of the image sensor. Image acquisition with a color image sensor and subsequent computation based on the weak object transfer function allow for quantitative amplitude and phase measurements of a specimen. We describe computational model and single-shot quantitative phase imaging capability of our method by presenting phase images of calibrated phase sample and dynamics of cells. Phase measurement accuracy is validated with pre-characterized phase plate, and single-shot phase imaging capability is demonstrated with time-lapse imaging of cells acquired at 30 Hz.
Wonchan Lee, Daeseong Jung, and Chulmin Joo, "Single-exposure quantitative phase imaging in color-coded LED microscopy (Conference Presentation)," Proc. SPIE 10074, Quantitative Phase Imaging III, 1007408 (Presented at SPIE BiOS: January 29, 2017; Published: 24 April 2017); https://doi.org/10.1117/12.2251746.5380600159001.
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