Limited-angle optical diffraction tomography (LAODT) is a powerful tool for measuring 3D refractive index distribution in biological microsamples. However, when thick objects are measured, reconstructions are erroneous due to diffraction errors even in the case when tomographic reconstruction algorithms take into account this phenomenon. We propose a hardware-based solution which allows to change a focal plane position with a liquid tunable lens in LAODT system. For each illumination angle, projections with different focal plane positions are recorded, and thus diffraction errors in the neighborhood of these planes are minimized. In this paper, we describe a method for processing data from a varifocal tomography setup that utilizes a Generalized Total Variation Iterative Constraint algorithm.
Wojciech Krauze, Arkadiusz Kuś, Ewa Skrzypek, and Małgorzata Kujawińska, "Reconstruction method for extended depth-of-field limited-angle tomography," Proc. SPIE 10074, Quantitative Phase Imaging III, 100741D (Presented at SPIE BiOS: January 31, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2251842.
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