Biopsies are conventionally performed in two dimensions. Histological slices in general present some micrometers in thickness, allowing that some molecular domains stay out of the resulting image. Thus the histopathological assay potentially is based on an incomplete set of information. The use of quantum dots as fluorescente probes allows the investigation of labelling pattern and biomarkers expression, along the three-dimensions of fresh histological slices, leading to more precise results. Present work show and discuss pattern and fluorescence intensity emission at the visible region obtained as a function of tissue thickness in histological (thickness(z)=7.6μm) breast cancer samples labeled with compact (7-10 nm) water soluble quantum dots. Series of 154 three-dimensional (3D) images were recorded from each tissue sample by laser scanning confocal microscopy, using 488 nm excitation.. In order to compare the results obtained, all the acquisition parameters were maintained constant. Results point to the possibility of more accurate histological diagnostics, once they clearly show distinct labeling patterns across sample thickness.
Patricia M. A. Farias, Arnaldo C. D. S. Andrade, Raquel Milani, Yolice P. M. Ruiz, Terezinha Tabosa, André Galembeck, and Andreas Stingl, "Quantum dots pushing up in vitro diagnostics limits," Proc. SPIE 10077, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV, 1007708 (Presented at SPIE BiOS: January 31, 2017; Published: 22 February 2017); https://doi.org/10.1117/12.2263761.
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