Laser-nanoparticles interactions have been widely used for several years. In biomedicine, several in vitro and in vivo experiments have shown promising results for the detection and treatment of cancer. One of the techniques of interest to us, is the nanoparticle-assisted photothermal therapy (PTT), which consists of irradiating cancer cells incubated with nanoparticles with either a pulsed or continuous (cw) laser in order to damage the cells.
However, there is still a debate over which type of laser is most effective for PTT for cancer treatment. On the one hand, cw lasers are minimally invasive and can be used for both detection and treatment of tumors. On the other hand, pulsed lasers offer great spatial precision and can deposit higher energy fluences than cw lasers, making them very efficient for inducing cavitation to damage cancer cells and tumors mechanically.
The aim of this study is to investigate whether simultaneous application of cw and pulsed laser could offer a synergetic enhancement of PTT efficacy to damage cancer cells in vitro, compared to either laser applied individually. PTT efficacy is evaluated through cell viability tests following the irradiation of prostate cancer (PC3) cells incubated with gold nanorods (5.7 X10 10 p/ml).
By irradiating the PC3-nanorod solution with the cw laser at 808 nm for ~60 seconds, the temperature increases from 37.5 to ~45°C, which damages some cancer cells via the heat shock response within the cells, and also could increase their sensitivity to the mechanical stress caused by the shock wave generated from inducing cavitation in the solution by the pulsed laser irradiation.
Noe Zamora-Romero, Guillermo Aguilar, Luis F. Devia-Cruz, Darren Banks, Bin Zhang, and David L. Halaney, "Photothermal therapy to damage PC3 cancer cells: in vitro studies of a pulsed laser (Conference Presentation)," Proc. SPIE 10078, Colloidal Nanoparticles for Biomedical Applications XII, 100780F (Presented at SPIE BiOS: January 29, 2017; Published: 24 April 2017); https://doi.org/10.1117/12.2252943.5381802541001.
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