Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE-1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.
Struan Bourke, Laura Urbano, Antoni Olona, Ferran Valderrama, Lea Ann Dailey, and Mark A. Green, "Silica passivated conjugated polymer nanoparticles for biological imaging applications," Proc. SPIE 10079, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX, 100790A (Presented at SPIE BiOS: January 30, 2017; Published: 21 February 2017); https://doi.org/10.1117/12.2252035.
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Monte Carlo based light propagation models to improve efficacy of biophotonics based therapeutics of hollow organs and solid tumours including photodynamic therapy and photobiomodulation (Conference Presentation)