We present an entirely fiber based laser source for non-linear imaging with a novel approach for multi-color excitation. The high power output of an actively modulated and amplified picosecond fiber laser at 1064 nm is shifted to longer wavelengths by a combination of four-wave mixing and stimulated Raman scattering. By combining different fiber types and lengths, we control the non-linear wavelength conversion in the delivery fiber itself and can switch between 1064 nm, 1122 nm, and 1186 nm on-the-fly by tuning the pump power of the fiber amplifier and modulate the seed diodes. This is a promising way to enhance the applicability of short pulsed laser diodes for bio-molecular non-linear imaging by reducing the spectral limitations of such sources. In comparison to our previous work [1, 2], we show for the first time two-photon imaging with the shifted wavelengths and we demonstrate pulse-to-pulse switching between the different wavelengths without changing the configuration.
Matthias Eibl, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Torben Blömker, and Robert Huber, "Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging," Proc. SPIE 10083, Fiber Lasers XIV: Technology and Systems, 100831C (Presented at SPIE LASE: February 01, 2017; Published: 7 March 2017); https://doi.org/10.1117/12.2251965.
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