We present the results of fabricating micro-structured macro-constructs for tissue engineering applications by using direct laser writing in pre-polymers (DLW-PP). The limiting factors for rapid construction of millimeter sized scaffolds, including the choice of material, software and hardware, as well as geometry, are discussed. We demonstrate that by using the hybrid inorganic-organic pre-polymer SZ2080 and employing a laser system which can combine the movement of linear motion stages with scanners, the structuring throughput reaching up to 51300 μm3/s can be achieved maintaining structural elements with 15 μm resolution. This allows to construct up to 30 scaffolds overnight with measurements reaching 1515×1515×195 μm3 – big enough for a surgeon to handle – and enables serial fabrication of such structures required for statistical tissue formation studies. Also, the versatility of DLW-PP technique is demonstrated by presenting the fabricated scaffolds with different pore shapes and sizes as well as manufactured out of pure and photo-sensitized SZ2080 material.
Sima Rekštytė, Justinas Mačiulaitis, Romaldas Mačiulaitis, and Mangirdas Malinauskas, "Fabrication of 3D micro-structured scaffolds by direct laser writing in pre-polymers for in vitro and in vivo studies," Proc. SPIE 10094, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII, 1009403 (Presented at SPIE LASE: January 29, 2017; Published: 17 February 2017); https://doi.org/10.1117/12.2249579.
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