The talk discusses recent technological progress in fabrication of NV fluorescent nanodiamond probes for intracellular sensing. Highly luminescent nanodiamond can be fabricated, by electron or proton irradiation. This includes irradiation using liquid targets yielding high homogeneity in concentration of NV centers over the irradiation batch. The surface functionalization methods, including selective substitution of surface groups by fluorine, stabilize NV- luminescence and provide probes, sensitive to NV0 - NV- charge switching. The charge sensing, using NV0 - NV conversion is further explained and used as sensitive method for detection of charge molecules and used for real time monitoring of DNA delivery. Finally prospects for using NV diamond probes, including particles with size < 10 nm for intracellular sensing, are discussed.
Milos Nesladek and Michal Gulka, "Fluorescent diamond probes: charge sensing and intracellular delivery (Conference Presentation)," Proc. SPIE 10118, Advances in Photonics of Quantum Computing, Memory, and Communication X, 1011802 (Presented at SPIE OPTO: January 31, 2017; Published: 2 June 2017); https://doi.org/10.1117/12.2256709.5397361499001.
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