Microscopy imaging optics can capture the high-resolution image at a certain focus plane, but the information outside that focal plane will become a blur and the information will be lost. We can adjust the optics stop or manually adjust the focal length, but the resolution will be reduced and it is capable of observing a high speed moving target in vivo. When a transparent plate was placed in front of a camera, the focusing point of the original system would be shifted. We proposed a variable focus system for extending the depth of field of the microscopy imaging system.
Lihui Wang, Tomohiko Hayakawa, and Masatoshi Ishikawa, "Depth of field extended imaging method based on intensification of time and spatial expansion," Proc. SPIE 10167, Nanosensors, Biosensors, Info-Tech Sensors and 3D Systems 2017, 101670C (Presented at SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring: March 26, 2017; Published: 17 April 2017); https://doi.org/10.1117/12.2259891.
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