From Event: SPIE Commercial + Scientific Sensing and Imaging, 2017
Single cell analysis can aid the study of molecular events responsible for cellular functions and unveil their connections to the biological functions of an organism. Biosensors based on surface enhanced Raman spectroscopy (SERS) can be used to this end and offer several advantages over other sensing platforms, such as sensitivity and multiplexed capabilities, among others. While SERS nanosensors/nanoparticles have been used for analysis in single cells, the delivery of such biosensors relies on cellular uptake, which requires long incubation time and has different efficiencies among cell lines. Nanosensors based on tapered optical fibers, instead, can be inserted in single cells and detect target molecules in subcellular compartment. The combination of these sensing devices with the transduction mechanism of nucleic acid based nanoprobes (i.e. inverse molecular sentinels) will permit the more direct detection of nucleic acids within single cells. This paper presents the development of tapered fiber-based biosensors for the detection of nucleic acid biomarkers in plant cells. The use of inverse molecular sentinels in plant cell was demonstrated. Sensors based on tapered fibers were fabricated and used to measure SERS from a single cell.
Pietro Strobbia, Bridget M. Crawford, Hsin-Neng Wang, and Tuan Vo-Dinh, "Nanosensors for nucleic acid targets detection using SERS," Proc. SPIE 10215, Advanced Environmental, Chemical, and Biological Sensing Technologies XIV, 1021508 (Presented at SPIE Commercial + Scientific Sensing and Imaging: April 09, 2017; Published: 3 May 2017); https://doi.org/10.1117/12.2267757.
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