From Event: SPIE Commercial + Scientific Sensing and Imaging, 2017
Off-axis incoherent digital holography that enables single-shot three-dimensional (3D) distribution is introduced in the paper. Conventional fluorescence microscopy images 3D fields by sectioning, this prevents instant imaging of fast reactions of living cells. In order to realize digital holography from incoherent light, we adapted common path configuration to achieve the best temporal coherence. And by introducing gratings, we shifted the direction of each light to achieve off-axis interference. Simulations and preliminary experiments using LED light have confirmed the results. We expect to use this method to realize 3D phase imaging and fluorescent imaging at the same time from the same biological sample.
Xiangyu Quan, Osamu Matoba, and Yasuhiro Awatsuji, "Analysis of off-axis incoherent digital holographic microscopy," Proc. SPIE 10219, Three-Dimensional Imaging, Visualization, and Display 2017, 102190U (Presented at SPIE Commercial + Scientific Sensing and Imaging: April 12, 2017; Published: 10 May 2017); https://doi.org/10.1117/12.2264217.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the conference proceedings. They include the speaker's narration along with a video recording of the presentation slides and animations. Many conference presentations also include full-text papers. Search and browse our growing collection of more than 12,000 conference presentations, including many plenary and keynote presentations.