In this work, we present successfully realization of a nonlinear microscope, not purchasable in commerce, based on stimulated Raman scattering. It is obtained by the integration of a femtosecond SRS spectroscopic setup with an inverted research microscope equipped with a scanning unit. Taking account of strength of vibrational contrast of SRS, it provides label-free imaging of single cell analysis. Validation tests on images of polystyrene beads are reported to demonstrate the feasibility of the approach. In order to test the microscope on biological structures, we report and discuss the label-free images of lipid droplets inside fixed adipocyte cells.
Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Vitaliano Tufano, and Luigi Sirleto, "Implementation of stimulated Raman scattering microscopy for single cell analysis," Proc. SPIE 10228, Nonlinear Optics and Applications X, 102280S (Presented at SPIE Optics + Optoelectronics: April 25, 2017; Published: 16 May 2017); https://doi.org/10.1117/12.2266776.
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