In this work, we present successfully realization of a nonlinear microscope, not purchasable in commerce, based on stimulated Raman scattering. It is obtained by the integration of a femtosecond SRS spectroscopic setup with an inverted research microscope equipped with a scanning unit. Taking account of strength of vibrational contrast of SRS, it provides label-free imaging of single cell analysis. Validation tests on images of polystyrene beads are reported to demonstrate the feasibility of the approach. In order to test the microscope on biological structures, we report and discuss the label-free images of lipid droplets inside fixed adipocyte cells.
Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Vitaliano Tufano, and Luigi Sirleto, "Implementation of stimulated Raman scattering microscopy for single cell analysis," Proc. SPIE 10228, Nonlinear Optics and Applications X, 102280S (Presented at SPIE Optics + Optoelectronics: April 25, 2017; Published: 16 May 2017); https://doi.org/10.1117/12.2266776.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the conference proceedings. They include the speaker's narration along with a video recording of the presentation slides and animations. Many conference presentations also include full-text papers. Search and browse our growing collection of more than 14,000 conference presentations, including many plenary and keynote presentations.
Study of self-shadowing effect as a simple means to realize nanostructured thin films and layers with special attentions to birefringent obliquely deposited thin films and photo-luminescent porous silicon