Possible dose-rate effects in a plasmid DNA exposed to pulsed extreme ultraviolet (XUV) and soft x-ray (SXR) water window radiation from two different table-top plasma-based sources was studied. Dose delivered to the target molecule was controlled by attenuating the incident photon flux with aluminum thin foils as well as varying the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were analyzed using the agarose gel electrophoresis. Some additional bands were identified in gel electrophoretograms as results of a DNA cross-linking. They were inspected by atomic force microscopy (AFM). Yields of single- and double-strand breaks (Gy-1 Da-1) were determined as a function of incident dose rate. Both yields decreased with a dose rate increasing. The ratio of single- and double-strand breaks exhibited only a slight increase at elevated dose rates. In conclusion, complex and/or clustered damages do not seem to be initiated under these irradiation conditions.
Ludek Vyšín, Tomáš Burian, Egor Ukraintsev, Marie Davídková, Libor Juha, Michael E. Grisham, Scott C. Heinbuch, and Jorge J. Rocca, "Is there any dose-rate effect in breaking DNA strands by short pulses of extreme ultraviolet and soft x-ray radiation? (Conference Presentation)," Proc. SPIE 10236, Damage to VUV, EUV, and X-ray Optics VI, 102360C (Presented at SPIE Optics + Optoelectronics: April 24, 2017; Published: 21 June 2017); https://doi.org/10.1117/12.2271171.5463398563001.
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